Abstract
A flow-injection (FI) methodology using (2,2 -dipyridyl) ruthenium(II) [Ru(dipy)3 2+ ] chemiluminescence (CL) was developed for the rapid and sensitive determination of metoclopramide hydrochloride. The method is based on the CL reaction of metoclopramide with
Ru(dipy)3 2+ and KMnO4 in a sulfuric acid medium. Under the optimum conditions, a calibration graph was obtained over the concentration range 0.005–3.5 g ml−1 with a limit of detection (S/N = 2) of 1 ng ml−1 . The correlation coefficient was 0.99993 (n = 8) with a relative
standard deviation of 0.48% for 10 determinations of 1 g ml−1 of drug. The method was successfully applied to the determination of metoclopramide in pharmaceutical preparations and biological fluids after IP administration of 25 mg kg−1 dose to rats. The elimination
half-life was 2.5 ± 0.4 h.
© 2003 Published by Elsevier B.V.
Keywords: Metoclopramide; Chemiluminescence flow-injection; Potassium permanganate –Tris(2,2 -dipyridyl) ruthenium(II) reaction; Pharmaceutical
analysis; Biological fluids
[PDF] Flow-injection chemiluminescent determination of metoclopramide
A flow-injection (FI) methodology using (2,2 -dipyridyl) ruthenium(II) [Ru(dipy)3 2+ ] chemiluminescence (CL) was developed for the rapid and sensitive determination of metoclopramide hydrochloride. The method is based on the CL reaction of metoclopramide with
Ru(dipy)3 2+ and KMnO4 in a sulfuric acid medium. Under the optimum conditions, a calibration graph was obtained over the concentration range 0.005–3.5 g ml−1 with a limit of detection (S/N = 2) of 1 ng ml−1 . The correlation coefficient was 0.99993 (n = 8) with a relative
standard deviation of 0.48% for 10 determinations of 1 g ml−1 of drug. The method was successfully applied to the determination of metoclopramide in pharmaceutical preparations and biological fluids after IP administration of 25 mg kg−1 dose to rats. The elimination
half-life was 2.5 ± 0.4 h.
© 2003 Published by Elsevier B.V.
Keywords: Metoclopramide; Chemiluminescence flow-injection; Potassium permanganate –Tris(2,2 -dipyridyl) ruthenium(II) reaction; Pharmaceutical
analysis; Biological fluids
[PDF] Flow-injection chemiluminescent determination of metoclopramide
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